For proper FFPE tissue sectioning and placement.

Tissue Prep

CytAssist for FFPE

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.

Best practices for handling tissue samples and slides before and after sectioning. Guidance for removing hard coverslips from archived tissue slides.

Deparaffinize, stain, image, and decrosslink tissue.

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Refer to instrument-specific documents for seamless instrument operation.

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Sequence your Visium spatial library as per our provided recommendations.

Analyze your Visium spatial data using our pipelines and visualization tools.