Spatial Gene Expression for FFPE
What is Spatial Gene Expression for FFPE?
Visium Spatial Gene Expression measures the whole transcriptome of FFPE tissue sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.
Documentation
Find resources, from protocols to free-to-use analysis tools.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.
Best practices for handling tissue samples and slides before and after sectioning. Guidance for removing hard coverslips from archived tissue slides.
Deparaffinize, stain, image, and decrosslink tissue.
Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.
Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.
Refer to instrument-specific documents for seamless instrument operation.
For imaging hardware, general image acquisition and analysis guidelines, and example images.
Sequence your Visium spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.
For proper FFPE tissue sectioning and placement.
Deparaffinize, stain, image, and decrosslink tissue.
For imaging hardware, general image acquisition and analysis guidelines, and example images.
Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.
Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.
Sequence your Visium spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.
Datasets
Access free, publicly available data from a number of different sample types generated with Visium for FFPE spatial solutions
Videos
Videos for Visium CytAssist for FFPE are coming soon.
Q&A
Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.
Publications
Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.
